Preparation, characterization and in vitro anticancer testing of quercetin-loaded nanocochleates

S. A. Sonwane*, M. J. Chavan, D. P. Hase, D. S. Chumbhale, A. S. Ambare, Y. T. Bodakhe.
Department of Pharmacognosy, Amrutvahini College of Pharmacy, Sangamner 422608, Maharashtra, India.
* Corresponding author. Phone: 9766758577; E-mail address:


Background: The natural flavonoid quercetin has shown anticancer properties but it is in vivo administration remains challenging due to its poor aqueous solubility and extensive in vivo metabolism. This juncture demands an effective, controlled release formulation of quercetin would be a significant advance for the treatment of cancer.

Purpose: The objective of the current work was to prepare novel quercetin-loaded nanocochleates in order to improve its therapeutic efficacy and cytotoxicity in human breast cancer cell line MCF-7.

Methods: Quercetin-loaded nanocochleates was prepared by a trapping method. The optimized quercetin-loaded nanocochleates were evaluated for size, entrapment efficiency, in vitro quercetin release, cytotoxicity study.

Results: Stable rolled-up layers as well as a tubular structure of nanocochleates possessing particle size and encapsulation efficiency of 180 ± 3 nm and 76.69 ± 3.21%, respectively were obtained. Nanocochleates demonstrated sustained release of quercetin at physiological pH. A significant improvement in vitro anticancer towards human breast cancer MCF-7 cells was observed.

Conclusion: Developed nanocochleates markedly improved the anticancer efficacy of quercetin. The nanocochleates technology would facilitate the administration of this flavonoid in the clinical setting.

Keywords: Quercetin, soya phospatidylcholine, nanocochleates, anticancer.



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